Hyperresponsive Platelets and a Reduced Platelet Granule Release Capacity Are Associated with Severity and Mortality in COVID-19 Patients.

BACKGROUND: Coronavirus disease 2019 (COVID-19) is often associated with mild thrombocytopenia and increased platelet reactivity. OBJECTIVE: The aim of the current study was to investigate the adenosine triphosphate (ATP) release kinetics of platelets in hospitalized SARS-CoV-2-infected patients. METHODS: We studied time-dependent platelet activation in whole blood by monitoring the ATP release kinetics upon stimulation with a PAR1 receptor agonist in 41 hospitalized critically ill COVID-19 patients, 47 hospitalized noncritically ill COVID-19 patients, and 30 healthy controls. RESULTS: Our study demonstrated that platelets of critically ill COVID-19 patients were hyper-responsive with a shorter platelet response time (PRT) and a reduced platelet granule release capacity (GRC), probably due to chronic activation. The median PRT of COVID-19 patients admitted to the critical care unit was 10 and 7 seconds shorter than the median PRT in healthy controls and noncritical COVID-19 patients, respectively. Both PRT and GRC were also associated with D-dimer (Spearman r [r s] = -0.51, p < 0.0001 and r s = -0.23, p < 0.05), C-reactive protein (CRP) (r s = -0.59, p < 0.0001 and r s = -0.41, p < 0.01), and neutrophil-to-lymphocyte ratio (NLR) (r s = -0.42, p < 0.0001 and r s = -0.26, p < 0.05). Moreover, an increased PRT and a reduced GRC were associated with an increased mortality (odds ratio [OR]: 18.8, 95% confidence interval [CI]: 6.5-62.8, p < 0.0001 and OR: 4.0; 95% CI: 1.6-10.4, p < 0.01). These relationships remained significant after adjustment for age, sex, D-dimer, CRP, and NLR. CONCLUSION: Using an accessible agonist-induced platelet granule ATP release assay, we show that platelet hyper-responsiveness and reduced platelet GRC in COVID-19 patients were associated with critical illness and mortality.

Determinant Factors of COVID-19 Vaccine Hesitancy Among Adult and Elderly Population in Central Java, Indonesia.

Purpose: The Coronavirus disease (COVID-19) vaccination program has been rolled out to address the pandemic. However, the COVID-19 vaccination coverage rate in Indonesia, especially in Central Java, is low. The study aimed to identify COVID-19 vaccine acceptance and to determine the factors associated with COVID-19 vaccine hesitancy. Participants and Methods: A cross-sectional study was conducted from September to October 2021. A self-reported questionnaire was distributed to participants aged ≥ 18 years and living permanently in the area of study by the multistage sampling technique. Bivariate and multivariate analyses were performed to determine the association. All statistical tests were significantly considered if the p-value <0.05 at 95% confidence interval (CI). Results: A total of 500 participants were eligible, with the age ranging from 18 to 76 years old. COVID-19 vaccine acceptance rate was 93.6%. In the multivariate logistic regression analysis, we found that the elderly (aOR=5.231; 95% CI=1.891-14.468), having comorbidity (aOR=4.808; 95% CI=1.975-11.706), not being exposed to information (aOR=7.039; 95% CI=2.072-23.908), not believing in the vaccine halalness (OR=3.802; 95% CI=1.272-11.364), not believing that vaccines could prevent the COVID-19 infection (OR=4.964; 95% CI=1.970-12.507), and having vaccination-related mild-moderate anxiety (OR=14.169; 95% CI=2.405-83.474) were more likely to have vaccine hesitancy (p<0.05). Place of residence, education level, belief that the vaccine could prevent the severe symptoms of COVID-19, and knowledge were significantly related to the vaccine acceptance in the bivariate analysis (p<0.05), but they were no longer significant in the multivariate (p>0.05). Conclusion: A high acceptance rate of the COVID-19 vaccine was found in this study. However, vaccine hesitancy is a major public health concern for attaining herd immunity and reducing the risk of case mortality. These findings could be the strategic focus for the government to improve COVID-19 vaccination coverage.

Pola pemberian antibiotik di era pandemi COVID-19 (studi kasus di rumah sakit nasional Diponegoro)

Inappropriate use of antibiotics could increase the incidence of antibiotic resistance hence increasing morbidity mortality and health cost. WHO has recommended to conduct a study of rational use of antibiotics in order to overcome this problem. Aim: To analyze the quality and quantity of antibiotic use in inpatient care facility at Diponegoro National Hospital (RSND). Methods: Quality and quantity assessment conducted by the use of DDD/100 patient/days and the Gyssens criteria. The study was conducted retrospectively at 86 patients’ medical records that were obtained by consecutive sampling method. Results: There were 141 antibiotics prescriptions from 86 medical records. Azithromycin levofloxacin and ceftriaxone were the most commonly used and had 4812;4401;and 2113 DDD/100 patient/days. By Gyssens criteria 354% of antibiotic usage was appropriate 511% of antibiotic usage was inappropriate and 135% no indication of the use of antibiotic. Based on the type of therapy ADE 894% and ADET 106%. Conclusion: In terms of quantity the most commonly used antibiotic was azithromycin. In terms of quality 354% antibiotic were used appropriately according to Gyssens criteria. Keywords: Antibiotic usage;internal medicine;DDD/100 patient/days;Gyssens criteria